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            您現在的位置:首頁 >> 產品中心 >> 生化試劑、抗體、血清 >> 常規生化試劑 >> Pharmcia17-0020-01Pharmcia(GE)葡聚糖凝膠G-15Sephadex G-15

            Pharmcia(GE)葡聚糖凝膠G-15Sephadex G-15

            • 更新時間:  2023-07-24
            • 產品型號:  Pharmcia17-0020-01
            • 簡單描述
            • Pharmcia(GE)葡聚糖凝膠G-15Sephadex G-15
              貨號:17-0020-02/17-0020-01
              品牌:Pharmacia
              產品名稱:葡聚糖凝膠G-15 17-0020-02/17-0020-01
              英文名稱:Sephadex G-15
              規格:25g/100
            詳細介紹

            Pharmcia(GE)葡聚糖凝膠G-15Sephadex G-15

            Sephadex G-15, 100 g

            Sephadex G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1 500 molecular weight.

            • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
            • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 1 500 molecular weight
            • Efficient removal of contaminants such as salts, dyes, and radioactive labels

             

            Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

             

            Pharmcia(GE)葡聚糖凝膠G-15Sephadex G-15

            Sephadex G-15, 100 g

            MatrixCross-linked dextran
            Particle Size Wet60 µm-180 µm
            Partical Size Dry40 µm-120 µm
            pH Stability Working Range2-13
            Flow Rate< Darcy´s Law1)
            Pressure/Flow Specification20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d.
            Fractionation [Mr] Globular Proteins1.5 x 103
            Fractionation [Mr] Dextrans>< 1.5 x 103
            Storage Conditions4 to 30°C
            AutoclavableIn wet form (pH 7.0) at 120°C for 30 min
            1)The beads behave as rigid spheres obeying Darcy's Law.

            Sephadex G-15, 100 g

            Handbook
            Size Exclusion Chromatography: Principles and Methods Summary

            This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close

            Magazine
            Upstream Downstream issue 2

            Close

            Material Safety Data Sheet
            Sephadex™ G-15, 100 g

            Close

            Sephadex™ G-15, 100 g

            Close

            Selection guide
            Protein and Peptide Purification: Technique selection guide Summary

            This technique selection guide is designed to help you in selecting the technique most suited to your application. It covers ion exchange chromatography (IEX), reversed phase chromatography (RPC), hydrophobic interaction chromatography (HIC), gel filtration (GF), and affinity chromatography (AC). Close


            Pharmcia(GE)葡聚糖凝膠G-15Sephadex G-15 詳細產品信息可和選購


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